Clinical Microbiology Procedures Handbook

Section 15: Biohazards and Safety

15.3 Biohazard Containment

Last Updated:2016
15.3.1 Introduction

In addition to implementing standard microbiological procedures and practices, management of the biohazards associated with working with pathogens includes physical barriers and administrative con trols. Physical barriers include primary safety…

15.3.2 Biosafety Levels

Biosafety levels (BSLs) are guidelines that describe appropriate containment equipment, facilities, and procedures for use by laboratory workers. The BSLs range from BSL 1 to BSL 4. Each BSL is…

15.3.3 Biological Safety Cabinet

The biological safety cabinet (BSC) is the most important primary containment equipment in the clinical microbiology laboratory. Class IIA laminar-flow cabinets are the most commonly used for BSL 2 and…

15.3.4 PPE and Engineering Controls

Observe standard precautions. OSHA standards for exposure to blood-borne pathogens require the laboratory to provide PPE for its employees.…

Section Editors: Gerald A. Denys, Larry D. Gray, and Ryan F. Relich

Editor in Chief: Amy L. Leber

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