Clinical Microbiology Procedures Handbook

Section 9: Parasitology

9.7 Sputum, Aspirates, and Biopsy Material

Last Updated:2016
9.7.1 Expectorated Sputum: Direct‐Mount and Stained Preparations

If the lab is not alerted to potential involvement of parasites, specimens will likely be routed to cytology and bacteriology and receive standard Papanicolaou, Giemsa, and Gram stains, resulting in the occasional surprise finding…

9.7.2 Aspirates and Bronchoscopy Specimens

The examination of aspirated material, bronchoalveolar lavage (BAL) fluid, and bronchial washings is useful when noninvasive sampling fails to demonstrate organisms. If parasite involvement…

9.7.3 Biopsy Specimens

This procedure provides instructions for performing impression smears (multiple parasites), teased preparations of skin for skin microfilariae, squash preparations of muscle for Trichinella, and skin scrapings…

9.7.4 Special Stain for Cestode Hooklets: Modified Trichrome-Ryan Blue

The diagnosis of larval cestode infections (primarily hydatid disease) often depends on the use of invasive procedures and subsequent examination of aspirate or biopsy specimens. Although slides prepared from these…

Section Editors: Robyn Y. Shimizu and Lynne S. Garcia

Editor in Chief: Amy L. Leber

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