Manual of Clinical Microbiology


Susceptibility Test Methods: General Considerations

Last Updated:2023

Determination of the antimicrobial susceptibilities of significant bacterial isolates is one of the most important functions of the clinical microbiology laboratory. From the physician's pragmatic point of view, the results…

Selecting an Antimicrobial Susceptibility Testing Method

Clinical microbiology laboratories can choose from among several conventional and novel methods for the performance of routine antibacterial susceptibility testing. These test methods include the broth microdilution, disk diffusion, antimicrobial…

Selecting Antibacterial Agents for Routine Testing

The laboratory has the responsibility to test antimicrobial agents and report those that are most appropriate for the organism isolated, the site of infection, and the clinical practice setting in…

Establishing Susceptibility Breakpoints

There is general agreement that the MIC is the most basic laboratory measurement of the activity of an antimicrobial agent against an organism. It is defined as the lowest concentration…

Molecular Detection of Resistance

As highlighted in chapter 80 of this Manual, there is now a range of molecular techniques for the detection of many resistance genes. Examples include the detection of…

Selected Use of Confirmatory and Supplementary Tests

Besides performing routine susceptibility testing, laboratories encounter isolates or test results which are unexpected, for which there is no testing guidance available, or which are considered to be of major…

Reporting of Results

The reporting of results is the crucial final step in susceptibility testing. There are no universally agreed‐upon practices for generating reports, but the following elements should be considered…

Role of the Laboratory in Antimicrobial Stewardship

In recent years, there has been an increasing emphasis on approaches to control and limit antimicrobial resistance in bacteria and fungi. The microbiology laboratory plays a central role in these…

Future Directions and Needs in Antimicrobial Susceptibility Testing

Antimicrobial resistance is becoming widespread among a variety of clinically significant bacterial species (75). Therefore, the microbiology laboratory plays a key role in the patient management process by…

Section Editor: Jean B. Patel

Volume Editor: James Karlowsky

Editors in Chief: Karen C. Carroll and Michael A. Pfaller

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